|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Misexpression of a Gli3ZnF-VP16 activator form of Gli3 can
influence limb positioning. (A) Schematic of full-length Gli3 protein (Gli3)
and a Gli3ZnF-VP16 activator form. Full-length Gli3 protein contains an
N-terminal repression domain (RD), a zinc finger DNA-binding domain (ZnF), and
an activation domain in the C-terminus (Act. Domain). The Gli3 activator form
contains the ZnF fused with two VP16 activation domains. (B-E) Dorsal views of
the embryo; injected limb is on the right. (B) Whole-mount in situ
hybridization with MyoD indicates the somites and Shh marks
the ZPA. There is a one-somite level shift in limb position (compare level of
left and right bars) following misexpression of Gli3ZnF-VP16 in the limb. (C)
The anterior border of the Tbx3 (C) and dHand (D) expression
domains in the forelimb region at stage 17 have been displaced rostrally
(arrowheads) following Gli3ZnF-VP16 misexpression. (E) Gli3
expression is downregulated following misexpression of Gli3ZnF-VP16
(arrowhead).
|
