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Fig. 1. ZLI differentiation in explants. (A-I) Hemisected forebrains viewed from the ventricular surface showing that the dorsal progression of ZLI differentiation in ovo (A-C) is recapitulated in forebrain explants cultured in vitro (D-I), as assessed by whole-mount in situ hybridization with probes for Shh (dark blue) and Lfng (red; A-F), and Ptc2 (G-I). (J) The length of the ZLI as a fraction of the total length of the alar plate (relative ZLI length) was assessed using Shh to mark the ZLI and Lfng to mark the dorsal boundary of the alar plate. (K) Quantitation of ZLI growth rates in ovo (blue line) and in vitro (red line). Error bars indicate s.e.m.





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