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Fig. 2. The ZLI forms from cells in the diencephalic alar plate. (A) Strategy to
ascertain whether cells in the basal plate contribute to the ZLI. (B-H) The
relative positions of labeled cells along the dorsoventral axis of the
diencephalon were assessed immediately following DiI injection (relative
initial position in B, and C,F) and after 48 hours in culture (relative final
position in B, and D,G). DiI was photo-converted to a brown precipitate and
analyzed in relation to the ZLI, as marked by Ptc2 expression (E,H).
Cells labeled in the ZLI (B, black circles) and elsewhere in the diencephalon
(B, gray circles) remained at the same relative dorsoventral position during
the experimental period. (C-E) DiI injected in the basal plate was never
observed in the ZLI (n=24). (F-H) Labeling in the ZLI observed after
focal injection of DiI into the alar plate (n=11).
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