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Fig. 6. Disruption of the adherens belt perturbs IrreC-rst localisation. (A-C) Clones homozygous mutant for shg^IH in a retina at 26% p.d., identified by the absence of DE-cadherin staining (green). IrreC-rst (violet) is not localised and appears as diffuse staining in the cytoplasm, while in the region heterozygous for shg^IH it colocalises with DE-cadherin at the 1°/IOC border. (D-F) GMR-Gal4<UAS-CAD retina at 22% p.d. exhibits a patchy distribution of -catenin (green) in the apical regions of the cells and diffuse staining in the cytoplasm. IrreC-rst (violet) is colocalised with -catenin at the 1°/IOC borders, but is also found at IOC/IOC borders. (G-I) In a GMR-Gal4<UAS-Rho^N19 retina at 18% p.d. the continuous adherens junctions, as revealed by staining for DE-cadherin (green), are eliminated. Single cells or ommatidia are difficult to discern and only minor speckles of immunoreactivity can be detected in the plasma membranes of IOCs and primary pigment cells. The majority of the protein appears as diffuse staining in the cytoplasm. IrreC-rst (violet) adopts an identical pattern. [Flies raised at 18 (shown here) or 25°C die as pharate adults with severe head defects and very rough eyes that are only one-third the size of wild-type eyes.] (J-L) Notch^ts1 discs heat-shocked at 19% p.d. In the absence of Notch function during cell sorting, IrreC-rst (violet) becomes ubiquitously distributed on all plasma membranes. Only partial cell sorting has taken place, leaving two rows of IOCs between ommatidia in many cases. DE-cadherin (green) is localised in a continuous apical belt but now also colocalises with IrreC-rst on all plasma membranes.

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