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Fig. 1. Timecourse of zebrafish brain ventricle formation. (A-K) Ventricles were
visualized by microinjecting a fluorescent dye, Texas Red-dextran, into the
hindbrain ventricle of anesthetized embryos. (A) Ventricle injection
schematic: lateral view of 24 hpf embryo with microinjection needle at
injection site of hindbrain ventricle. (B-K) Developmental profile of brain
ventricle morphology at 18, 20, 22, 24, and 30 hpf following dye injection;
(B-F) dorsal views; (G-K) lateral views; anterior to left. Heartbeat onset at
24 hpf (E), after brain ventricles have formed. (L-O) Cell morphology of
hindbrain ventricle was visualized by confocal microscopy after overnight
immersion in fluorescent molecule bodipy ceramide. (L) Diagram of lateral view
of 18 hpf embryo, with horizontal plane used for confocal time-lapse imaging
indicated by green line. (M-O) Confocal time-lapse imaging of hindbrain
ventricle of living, anesthetized embryo, beginning at 19 hpf and ending at 20
hpf. Asterisks label hinge-points from which opening begins, and arrows point
to locations of apparent adhesion that release as ventricle opens anterior to
posterior. Asterisks: midbrain and hindbrain hinge-points. Scale bar: 50
µm. A, anterior; F, forebrain ventricle; H, hindbrain ventricle; M,
midbrain ventricle; P, posterior.
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