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Fig. 8. {alpha}-Na+K+ ATPase localization in nok mutants and Nok localization in snk mutants appears normal. (A,B) a6F antibody labeling (green) at 24 hpf in horizontal section through midbrain and hindbrain of wild type (A) and nok mutant (B) with phalloidin-Texas Red as counterstain (red). Note that while the nok mutant hindbrain ventricle is severely reduced, a6F antibody still labels apical membrane. (C,D) Nok antibody labeling (green) at 24 hpf in horizontal section through midbrain and hindbrain of wild type (C) and snk mutant (D), with phalloidin-Texas Red as counterstain (red). Nok localizes at the apical membrane in both wild type (C) and mutant (D), suggesting that Snk is not necessary for Nok targeting to apical surface. Note: the snk mutant does have normal hinge-points in the midbrain and hindbrain, although they are not present in the optical section shown in D. Scale bar: 50 µm.





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