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Fig. 3. even-skipped expression in the posterior growth zone and germband.
Germband stage embryos are stained for even-skipped (purple) and
engrailed (orange). (A1) Early germband with Of'eve
accumulation in growth zone. Third thoracic segment is marked (T3). The growth
zone is large. (A2) Higher magnification of embryo shown in A1, showing
appearance of first abdominal Of'en stripe, coincident with a stripe
of Of'eve. (B) Mid-germband stage embryo stained only for
Of'eve. The midline region of the early stripe is still contiguous
with the growth zone patch, indicating that this stripe is still in the
process of leaving the growth zone. (C1) Mid-germband stage embryo.
Of'eve expression is maintained in the growth zone and in three
stripes just anterior to growth zone expression. Growth zone is now smaller
than in A1. (C2) Higher magnification of embryo shown in B1, showing
coincident expression of Of'eve and Of'en in three early
segments. Of'eve is most strongly expressed in the younger (more
posterior) segments, while Of'en is expressed more strongly in older
(more anterior) segments. Thus, Of'eve expression is fading just as
Of'en expression initiates. (D1) Embryo at end of germband
elongation. Growth zone expression of Of'eve fades. (D2) Higher
magnification of embryo shown in D1. Final Of'eve stripe is
coincident with tenth abdominal Of'en stripe. Remaining posterior dot
of Of'eve may represent anal ring expression. (E) Cartoon showing
birth and progressive maturation of Of'eve germband stripes. Early
stripes seem to `peel' away and are often contiguous with growth zone patch.
Scale bars: 200 µm for A1,C1,D1; 100 µm for A2,B,C2,D2.
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