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Fig. 3. AS MO knockdown of injected mRNA expression. (A,B) N teloblasts were
injected at stage 6 with nGFP mRNA, 3 hours later with a mixture of
fluorescent dextran (RDA) and antisense morpholino oligomer (AS MO), and then
imaged after another 46 and 98 hours. The left side of each panel shows the
combined RDA (red) and nGFP (green), and the right side shows nGFP only,
revealing the decrease in nGFP fluorescence in clones produced after the AS MO
injection. In these experiments, two or three blast cells were produced
between the first and second injections. (C) Enlarged view of the embryo shown
in A; fluorescence intensity was measured along the line shown crossing blast
cell nuclei produced before and after the AS MO injection and plotted on the
right. Scale bar: 10 µm.
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