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Fig. 5. Application of nGFP mRNA injections to analysis of the N lineage. (A) Time
series showing the anterior portion of an n bandlet; one ns blast cell was
uniquely labeled by tandem injections of the parent teloblast, first with nGFP
mRNA and then, after one blast cell was born, with RDA. Beginning 58 hours
after the first injection (I, 58-70), the uniquely labeled clone was imaged
hourly for 12 hours. During this time, the clone increased from two cells to
seven; a similar pattern of nuclei was observed in a sibling embryo imaged
only at 70 hours cl.ag. (II, 70). (B) Reconstruction of an embryo injected as
in A at 86 hours cl.ag. In the first (ns) labeled clone, superficial nuclei
are pseudocolored lavender and deep nuclei (2, 4, 8, 11, 12, 14) are red (8
and 12 are obscured by overlying nuclei). In the next (nf) clone, nuclei are
pseudocolored green. Those in more posterior clones appear white. The numbers
over the nuclei of the ns and nf clones correspond to cells in the lineages
trees in Fig. 6A and B,
respectively. (C) Three-dimensional reconstruction of a similar embryo with
the ns clone at 90 hours cl.ag. The ns clone (lavender) now has 18 nuclei; the
posterior nf clone (green) has 18 interphase nuclei and one in mitosis (broken
outline); the exact identities of these cells remain to be determined. In this
panel, the cytoplasmic RDA fluorescence is shown in red surrounding nuclei in
the nf and more posterior (white) clones, so that the elongating fissure
between prospective ganglionic primordia is visible (arrow); midline towards
left in B and C. Scale bar: 10 µm.
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