Supplemental Figure 1
-
Fig. S1. Effects
of D/V axial rotation of the hindbrain alone on expression patterns of ventral
otic genes. (A) A schematic diagram showing DV rotation of the hindbrain alone at
E1.5 (10-13 ss). The notochord is
separated from the hindbrain and discarded before rotation. The rotated embryos
are processed as described in Fig. 6. (B,C) Shh expression
pattern in control (B) and hindbrain-rotated (C) embryos. (D-G) Expression
patterns of Six1 and Lfng in control (D,F) and hindbrain rotated (E,G) embryos
(arrows). Six1 and Lfng expression domains are shifted dorsally in hindbrain
rotated embryos (arrows; E,G). Scale bars: in B, 100 mm for
B,C; in D, 100 mm
for D-G.