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Fig. 3. The AP axis of the inner ear is not affected by AP axial rotation of the hindbrain. (A-D) Lfng expression patterns in control (A,B) and R4-R7 rotated (C,D) embryos at E2.5. Arrows indicate the normal Lfng expression patterns. (E-H) NeuroD expression patterns in control (E,F) and R4-R7 rotated (G,H) embryos. Arrows indicate the normal NeuroD expression in the otic epithelium as well as delaminated neuroblasts. (I,J) Anatomy of the inner ear after rhombomere rotation (J) is similar to controls (I) at E7. AA, anterior ampulla; ASC, anterior semicircular canal; CC, common crus; CD, cochlear duct; ED/S, endolymphatic duct and sac; LA, lateral ampulla; LSC, lateral semicircular canal; PA, posterior ampulla; PSC, posterior semicircular canal. Scale bars: in A, 100 µm for A,C,E,G; in B, 100 µm for B,D,F,H; in I, 500 µm for I,J.





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