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Fig. 6. Effects of DV axial rotation of the hindbrain/notochord on expression
patterns of ventral otic genes. (A) DV rotation of the hindbrain and notochord
between R4 and R7 at E1.5. (B,C) Shh expression pattern in
whole-mount of control (B) and DV rotated hindbrain (C) embryos. (D,E)
Shh-positive floor plate and notochord, which are normally located
ventrally in control (D), are located dorsally in hindbrain rotated embryos
(E, arrow). Red lines in B and C indicate the level of sections shown in D and
E, respectively. (F-M) Expression patterns of NeuroD, Lfng, Six1 and
Otx2 in control (F,H,J,L) and hindbrain rotated (G,I,K,M) embryos.
NeuroD (G), Lfng (I) and Six1 (K) are downregulated
in their normal expression domain ventrally and upregulated in the
dorsoanterior aspect of the otocyst (arrows). (L,M) Otx2 expression
is expanded dorsally (M) without the loss of its normal ventral domain (L)
(brackets). An asterisk indicates the NeuroD-positive neuroblasts.
Scale bars: in B, 100 µm for B,C; in D, 100 µm for D,E; in F, 100 µm
for F-M.
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