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Fig. 2. Identification of an enhancer to drive lacZ expression in the
dorsal spinal cord. (A) Schematic representation of the genomic structure of
Mbh1 and Mbh1/lacZ transgenes. The orange boxes
indicate exons, and translation start site (ATG) is designated as nucleotide
number 1. The blue and green boxes represent lacZ and the
ß-globin promoter/lacZ construct (BGZA),
respectively. The 3' fragment was reversed in Tg6. The number of
transgenic (#TgM) and ß-gal+ (#expression) embryos are shown
in the table; athe two embryos expressed lacZ at low
levels in the midbrain. Lateral (B) and dorsal (C-E) views of representative
ß-gal+ embryos harboring Tg2 (B,C), Tg4 (D) and Tg5 (E).
Transverse sections of the embryos carrying Tg2 (F) and Tg4 (G). Arrowheads
indicate ß-gal+ ventral funiculi. (H) Transverse section of
the embryo carrying Tg4 was immunostained with antibodies against Math1 (red)
and ß-gal (green). Scale bars: in B, 1 mm for B-E; in F, 100 µm for
F,G; in H, 50 µm.
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