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Fig. 4. Math1 was necessary for Mbh1 expression in the spinal
cord. Transverse sections at brachial (A-C,E-G) and forebrain levels (D,H) of
E11.5 Math1+/+ (A-D) and
Math1/ (E-H) mouse embryos were hybridized
with antisense cRNA probes for Math1 (A,E), Mbh1 (B,D,F,H)
and Lhx9 (C,G). Loss of Mbh1 and Lhx9 expression
was observed through all axial levels of the spinal cord in all
Math1/embryos (n=3). Scale bar: 100
µm.