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Fig. 7. Transcriptional activation by Math1 in the spinal cord. The E11.5 spinal
cord was electroporated with Tg12 and either EYFP (A-D) or
EYFP/Math1 (E-H), and stained with X-gal, two days after
electroporation. (A,B,E,F) Dorsal views of the spinal cord. (C,D,G,H)
Transverse sections at brachial levels. Dark (A,C,E,G) and illuminated
(B,D,F,H) views to show transfected (EYFP+) and
ß-gal+ cells, respectively. Upper (in A,B,E,F) and right (in
C,D,G,H) sides were transfected with the genes. Arrow, ß-gal+
cell; arrowheads, commissural axons. Scale bars: in A, 1 mm for A,B,E,F; in C,
100 µm for C,D; in G, 100 µm for G,H. (I) Quantitative analysis of
transcriptional activation by Math1. Two days after electroporation, cell
extracts were prepared from EYFP+ portions. After normalization to
EYFP fluorescence, the data are expressed as ß-gal+ activity
relative to the activity obtained by transfection of EYFP alone.
Error bars indicate standard error of at least three independent
experiments.
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