(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.
Fig. 1. Molecular analysis of vls. (A) Location of three EMS mutations in
vls. All mutations lead to a premature stop codon. WD repeats are
shown in grey. (B) The vls transcript and restriction map of the
genomic DNA used for generating a vls rescue transgene. Exons are
drawn as boxes and the putative open reading frame is indicated in black. B,
BamHI; Bs, BstEII; P, PstI; R, EcoRI. (C)
Expression of the P[HA-Vls] transgene in ovaries revealed by western
blotting using anti-HA antibodies. A band of 
50 kDa is detected in
transgenic but not in wild-type ovaries. (D) Alignment of the amino acid
sequences of Vls and homologous proteins: CP8824, an incomplete Anopheles
gambiae homolog; human MEP50; and MGC65780 a zebrafish protein. These
proteins display strong conservation of the WD repeats (grey boxes). MEP50 and
the zebrafish homolog display six potential WD repeats, whereas
Drosophila Vls contains only four such repeats, as predicted by the
Protein Sequence Analysis server of the BioMolecular Engineering Research
Center of Boston University
(http://bmerc-www.bu.edu/psa/).
Multiple sequence alignment of Vls and related proteins was performed using
the Pileup program of the Wisconsin Package (Genetics Computer Group). Gaps in
the amino acid sequence, indicated by dots, were introduced for optimal
alignment. The GenBank accession numbers of these sequences are the following:
CP8824_AG, EAA14269 MEP50_HS, AAL79917 MGC65780_DR, AAH56278
