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Fig. 3. Vls is a component of nuage and pole plasm. Fixed, whole-mount egg chambers
stained with Oli-Green for DNA and anti-HA antibodies in homozygous
vls3 flies expressing P{HA-Vls} (A,C,E,F,H,I,K)
and in wild-type flies as controls (B,D,G,J,L). HA-Vls is detected at the
posterior pole of the oocyte in transgenic vls3; P{HA-Vls}
stage 10 egg chambers (A,C). (C,D) Enlarged views of similar stage 10 oocytes,
as shown in A and B, respectively. HA-Vls is observed at the posterior pole of
early vls3; P{HA-Vls} embryos and in pole cells (E,F,H,I).
(F,G,I,J) Enlarged views of similar syncytial and blastoderm embryos, as shown
in E and H, respectively. HA-Vls is detected in the nuage surrounding the
nurse cell nuclei (K).
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