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Fig. 7. Tud-binding domain in Vls. As performed in
Fig. 2C, full-length GST-Vls or
derivatives were incubated with S•Tag-Tud9A1-N. Bound S•Tag-Tud9A1-N
was detected as described in Fig.
6B. Input: one-tenth of the S•Tag-Tud9A1-N extract. The
smallest fragment of Vls showing binding to Tud9A1-N encompasses the first WD
domain shown in green. The amount of GST-Vls proteins was visualized by
Coomassie staining (lower panel). Representation of the GST-Vls fragments used
for mapping and summary of the results are indicated on the right panel.
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