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Fig. 4. Analysis of AP patterning defects in conditional COUP-TFII mutant
stomach. Controls and conditional mutant embryos were obtained by mating with
COUP-TFII floxed homozygous males and
Nkx3-2Cre/+; COUP-TFIIflox/+ females
as described in Fig. 3. (A,B)
Stomachs were dissected from E16.5 embryos and examined in whole mount under
the dissecting microscope. The presumptive margin between fore-stomach and
hind-stomach is indicated by dots and is shifted slightly anteriorly in the
mutant. (C,D) Whole-mount postnatal day 28 stomachs were dissected and
examined under the dissecting microscope. Position of limiting ridge is
indicated by arrowhead. A clear anterior shift of the limited ridge is
observed in the conditional mutant. (E,F) Dissected stomachs from E16.5
embryos were longitudinally sectioned and stained with Hematoxylin and Eosin.
(G-H) Higher magnification of similar regions as indicated by lines in E,F. e,
epithelium; sm, smooth muscle layer; fs, fore-stomach; hs, hind-stomach; oe,
esophagus; d, duodenum.
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