|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 9. Effect of inhibition of Hh signaling in COUP-TFII expression.
(A,B) Developing stomachs were dissected from E12.5 Shh wild-type and
null mutant, and expression of COUP-TFII mRNA was detected by
whole-mount in situ hybridization. Caudal margins of COUP-TFII
expression are indicated by dots, and presumptive anatomical boundaries
between stomach and duodenum are indicated by arrowheads. The relative size of
fore-stomach is indicated by a white line at the top of the stomach. (C-F)
Foregut explants were dissected from E10.5 knock-in embryos and cultured for
48 hours in increasing concentrations of cyclopamine (0-10 µM, D-F). Strong
lacZ expression was detected in the mesenchyme between lung bud and
fore-stomach, as indicated by arrowhead, and cyclopamine inhibited
lacZ expression in a dose-dependent manner. Scale bars: in B, 0.5 mm
for A,B; in F, 0.5 mm for C-F. oe, esophagus; fs, fore-stomach; hs,
hind-stomach; dmg, dorsalmesogastrium; lb, lung bud; St, stomach; Py, pylorus;
d, duodenum; e, hind-stomach epithelium.
|
