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Fig. 4. Expression of BOP1 and BOP2. (A,B) BOP2
expression in Col-0 wild type as determined by in situ hybridization. (A)
Vegetative shoot apex from an 11-day-old plant. BOP2 is expressed in
the incipient leaf primordia (ip) and in the proximal regions of emerging
leaves (arrowheads). Scale bar: 100 µm. (B) BOP2 expression in the
inflorescence apex. Expression can be seen in the incipient flower primordium
(fp) and in the proximal parts of developing flower organs (arrowhead). Scale
bar: 100 µm. (C-I) Expression of BOP1::GUS. (C-E) 5-day-old
seedling. (C) BOP1 expression can be found in the proximal parts of
leaf 1 and 2 and at the base of the cotyledons. (D) Histological section
showing BOP1 expression in the proximal margins of leaf primordia.
(E) BOP1 is expressed along the midveins of leaf 1 and 2, and along
the cotyledon petioles. (F,G) 10-day-old seedling. BOP1 is expressed
at the base of the petioles. In G, young leaves have been removed for clarity.
(H) Section of an inflorescence apex showing BOP1 expression in
proximal parts of developing flower organs. Scale bar: 100 µm. (I)
Inflorescence showing BOP1 expression overlapping the flower organ
abscission zone (az) and at the base of the pedicels (arrowhead). The base of
the indicated pedicel has been magnified (inset) to show the BOP1
expression.
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