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Fig. 3. Nuclear re-organisation of Hoxb1 during early embryogenesis. (A)
FISH with MMU11 chromosome paint (green) and Hoxb1 probe (red) on a
field of nuclei from E7.5 primitive streak regions. The image is a maximal
pixel intensity projection from a 3D image stack. (B) Single z-plane
images from FISH as in A, of single nuclei from E7.5 EEM and PSM. Nuclei were
counterstained with DAPI (blue). Scale bars: 2 µm. (C) Histograms showing
the position of Hoxb1 and Hoxb9 hybridisation signals,
relative to the inside, edge or outside of the MMU11 territory, in nuclei from
E7.5 EE cells (black bars) and PSM (white bars). The EE and PSM analysed were
from two and three embryos from different litters in the case of
Hoxb1, and from one embryo for Hoxb9. Negative distances
indicate signals localised beyond the visible limits of the detectable CT. For
Hoxb1, data from EEM and EEE have been pooled together
(n
60). (D) Position (mean±s.e.m.) of Hoxb1 (black
squares) and Hoxb9 (white circles) relative to the inside, edge or
outside of the MMU11 territory in nuclei from EE and PSM at E7.5
(n60).
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