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Fig. 6. Chromatin condensation and nuclear re-organisation of Hoxb in the E6.5 early streak embryo. (A) HE staining (left) and DAPI counterstaining (right) of serial transverse sections from an E6.5 embryo. The position of the posterior streak region (PSR), and the posterior (PEP) and anterior (AEP) epiblast are indicated. Scale bar: 200 µm. (B) The distribution of squared interphase distances (d2) measured between probes for Hoxb1 and Hoxb9, in PSR, DR, PEP and AEP of E6.5 embryos. Measurements from E7.5 EEM are shown for comparison. (C) Histogram showing the position of Hoxb1 hybridisation signals, relative to the MMU11 territory edge, in nuclei from E7.5 EE (black bars), E6.5 DR (white bars) and E6.5 PSR (grey bars). Negative distances indicate signals localised beyond the visible limits of the detectable CT (n≥60). (D) Position (mean±s.e.m.) of Hoxb1 (squares) and Hoxb9 (circles) relative to the MMU11 territory edge in nuclei from E7.5 EE and E6.5 PSR (n≥60).





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