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Fig. 3. Nok is the functional homolog of mammalian Pals1/Mpp5. (A) nok^s305 mutant with ventrally curved body form and severely disrupted retinal pigmented epithelium at 34-36 hpf. (B) Wild type. (C) Murine Pals1/Mpp5 mRNA results in a partial phenotypic rescue of nok^s305 mutants, including the body form. (D) Wild-type retinal pigmented epithelium. (E) The nok^s305 retinal pigmented epithelium is severely disrupted. (F) Retinal pigmented epithelial cells are coherent in Pals1 mRNA-injected nok^s305 mutants (partial rescue). (G) A summary table showing the percentages of embryos with peripheral circulation among nok morphants injected with 50 ng/µl wild-type nok or 50 ng/µl Pals1/Mpp5 mRNA.

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