|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. Expression of Abd-B and cad in the embryonic genital
disc. Anterior is to the top. (A-C) Double staining of a late stage
15 hdc-lacZ embryo with anti-ß-galactosidase (green, A, note the
three clusters) and anti-Abd-B (red, B) antibodies. A merged image is shown in
C. Note that the posterior cluster of genital disc cells is not marked with
Abd-B (arrow). (D-F) Double staining of a stage 15
cad-Gal4/UAS-GFP; hdc-lacZ/+ embryo marked with
anti-ß-galactosidase (red, D) and GFP (green, E). A merged image is shown
in F. See that the posterior cluster (arrow) shows cad expression.
(G-L) Embryonic genital disc cells of a
UAS-myc-EGFPF/+;
Abd-B-Gal4LDN/hdc-lacZ embryo, showing expression
corresponding to the Abd-B m transcript (GFP expression, green, I-L)
in the anterior lateral cells of the disc primordium, marked with an
anti-ß-galactosidase antibody (blue, G,J,L). GFP-marked cells are a
subset of those expressing Abd-B (red, H,K,L). Note that about four
anterior-lateral cells of the disc primordium express GFP (arrows, J) and that
some Abd-B-expressing cells are not marked with GFP (arrows, K). A
merged image is shown in L. vc, ventral cord.
|
