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Fig. 3. Expression and requirement of abd-A in the female internal genitalia. (A) Third instar female genital disc, stained with an anti-abd-A antibody. (B) Similar disc of an abd-A-lacZ larva stained with X-Gal. The pattern of expression reproduces that of the anti-abd-A antibody. (C,D) X-Gal staining of internal female genitalia from two late pupae. The seminal receptacle (sr), spermathecae (s), uterus (u) and oviducts (ov) are stained, whereas parovaria (p) are not. o, ovaries. (E) Female genital disc of an abd-A^iab3-277/DfR59 female larva stained with anti-abd-A antibody, showing a reduction in abd-A expression compared with wild type. (F) Female internal genitalia of an abd-A^iab3-277/Df 109 female. See that most elements of the internal genitalia and the ovaries have disappeared (compare with C). vp, vaginal plates (part of the external genitalia).

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