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Fig. 5. Abd-B m function in the genitalia and the female genital disc. (A) An Abd-BM5 mutant clone in the female right eighth hemi-tergite, marked with yellow, is converted into an anterior tergite (arrow). Compare with the left, wild-type, hemi-tergite (arrowhead). (B) Similar mutant clone showing transformation of the genitalia into a sternite (arrow). S7, seventh sternite. (C) Abd-BM3 clone, marked with yellow, showing transformation of the male A8 tergite (normally very small) into an anterior one (arrow). T7, seventh tergite; g, genitalia. (D-F) Abd-BM5 clones in the A8 of the female disc, marked by the absence of the lacZ marker (green, D), do not activate Dll (red, E; arrowheads in E and H mark the wild-type Dll expression). a, analia. The merged image is shown in F. (G-L) abd-AM1 mutant clones in the female A8, marked by the absence of the ß-galactosidase marker (G,J, green), do not eliminate Dll (H, red) or Abd-B (K, red) expression. Merged images are shown in I and L. (M-O) Abd-BM5 clones in the A8 of the female genital disc, marked by the absence of the lacZ marker (in green in M), do not change abd-A expression (red, N). A merged image is shown in O. (P-R) Abd-BD18 mutant clones in the A8 segment, marked by the absence of the ß-galactosidase marker (blue, P,R; clones are outlined), eliminate abd-A expression, detected by an anti-abd-A antibody (green, Q,R), and activate Dll protein expression (red, P,R). (S) Abd-BD18 abd-A+ clones, marked with GFP (in green), eliminate Abd-B (blue) and activate Dll (red) expression. The boxed clone is shown in detail in T-W. (X) Similar clones induced in the leg disc do not eliminate Dll, as shown by the co-expression of the GFP marker (green) and Dll (red), giving a yellow color (arrowheads). (Y) dpp-Gal4/UAS-Dll female disc showing elimination of abd-A expression in the dpp domain (arrowheads).





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