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Fig. 6. Abd-B expression in Abd-B m mutant embryos and clones, and Abd-B m expression and function in the female internal genitalia. (A) Wild-type stage 11 embryo showing Abd-B expression, detected with the lA2E9 monoclonal antibody, in PS12-PS14 (A7-A9). (B) Abd-B^M5 homozygous embryo at a similar stage, stained with the same antibody. The Abd-B expression is restricted to PS14. (C-E) Abd-B^M5 clones induced in female A8 do not eliminate Abd-B expression. Clones are marked by the absence of GFP marker (green, C), and Abd-B expression is detected with the anti-rabbit anti-Abd-B (red, D). A merged image is shown in E. (F) Abd-B^M5 clones induced in the same primordium, marked by the absence of X-gal staining (light blue), do not ectopically activate Abd-B r expression (purple). (G,H) Detail of the regions boxed in F. Note the Abd-B r wild-type expression in the female A9 (arrowheads) and its absence, or very low expression, in the clones (arrows). (I) Female internal genitalia of an UAS-lacZ/+; Abd-B-Gal4^LDN/+ late pupa. The spermathecae (s) and uterus (u) are stained, whereas parovaria (p) and oviducts (ov) are not. o, ovaries; vp, vaginal plates. (J) Detail of I, showing absence of staining in the parovaria. (K) Abd-B m mutant female showing disappearance of all the external and internal genitalia except the parovaria (p). h, hindgut; a, analia.

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