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Fig. 1. MTOC material and centrioles localize to the oocyte nucleus during
mid-oogenesis. In stage 6 (A) and stage 8 (B), 
Tub37C, (Rb1011,
red) is detected around the oocyte nucleus and as a dot close to it
(arrowheads in A,B). (A-C) DNA green. (C) Tub23C (R77, red) is
detected around the oocyte nucleus at stage 8. A similar distribution is
observed at stage 6 (data not shown). (D-G) Egg chambers expressing
GFP-cter-D-PLP. (D) 3D reconstruction of the oocyte nucleus. D-PLP is detected
as a dot (light blue, arrowhead) on the surface of the nucleus (inset,
original data). (E-G) D-PLP (single view in E, green) and Tub37C
(single view in F, red) co-localize (G) during stage 8 to the same dot close
to the nucleus (arrowheads). (H) Polyglutamylated MTs (ID5 antibody)
are detected as a dot in the vicinity of the nucleus during stage 9
(arrowhead, inset magnification view). (I,J) Electron micrographs of
the anterior dorsal corner of the oocyte; anterior is to the top, dorsal to
the right. (I) Low power magnification of the area around the oocyte nucleus.
The centrioles are found in the boxed area. (J) At high magnification, two
centrioles (white arrowheads) are visible, most probably there are two more
(black arrowheads). Golgi cisternae are in the vicinity of the centrioles
(arrow). (K) Egg chamber expressing D-PLP. The centrosome is found
close to the nucleus (arrow; oocyte outline indicated with dashed line).
(L-N) Flies fed with colchicine. (L) The nucleus is misplaced and
anti-Khc staining revealed MTs only in the follicle cells. The centrioles
(arrows) are separated from the oocyte nucleus in a stage 8 (M) or stage 9 (N)
oocyte expressing D-PLP. Asterisk, oocyte nucleus. Scale bars: 20 µm in
A-H,K-N; 1 µm in I,J.
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