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Fig. 2. Comparative analysis of MTs in living and fixed egg chambers. MTs in fixed stage-8 egg chambers detected with anti- ${alpha}$ -Tubulin-FITC (A) and anti-Khc (B) (inset in B specificity of the anti-Khc antibody, a Khc^7.288 mutant germline clone shows no anti-Khc staining in the oocyte). (C-E) Stage 7 egg chambers; (F-H) stage 9 egg chambers. (C,F) Living egg chambers expressing GFP- ${alpha}$ -Tubulin. (D,G) Fixed egg chambers stained with anti- ${alpha}$ -Tubulin-FITC. (E,H) Fixed egg chambers stained with anti-Khc. (I,J) Stage 9 egg chambers, Khc (red) and aromatic c-ter amino acid of ${alpha}$ Tub (green). The arrow in J points to the posterior Khc pool.

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