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Fig. 3. Extraction procedure is not affecting egg chamber polarity. (A) Surface view of a fixed stage-10 egg chamber stained for anti- ${alpha}$ -Tubulin-FITC. (B,C) MTs in stretched follicle cells revealed by anti- ${alpha}$ -Tubulin-FITC in fixed egg chamber (B) and by GFP- ${alpha}$ -Tubulin in living egg chamber (C). MTs in main body follicle cells revealed by anti- ${alpha}$ -Tubulin-FITC in fixed egg chamber (D) and by GFP- ${alpha}$ -Tubulin in living egg chamber (E). (F,G) Atypical protein kinase C. Posterior follicle cells fixed after a standard procedure using 4% paraformaldehyde (F) or after the extraction protocol (G). (H,I) Fixed oocyte expressing Kin::LacZ, stained for anti-Khc (red) and anti-ß-Gal (green) (arrow, posterior fraction of Khc). (J,K) Fixed oocyte expressing Nod::LacZ, stained for anti-Khc (red) and anti-ß-Gal (green) (Arrows, anterior distribution of Nod). Grk (L) and Osk (M) protein after the extraction protocol. Scale bar: 20 µm.

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