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Fig. 5. The nucleus is an active center for MT polymerization. (A)
Schematic representation of the experimental setup for cold-induced MT
disassembly. Complete depolymerization: dissected ovaries were extracted (30
minutes), placed on ice (30 minutes), fixed and analyzed for MTs by anti-Khc.
To allow MT regrowth, ovaries were incubated at 25°C for 3-30 minutes.
(B) Stage 9 oocyte, complete MT depolymerization. Khc was only
detectable at the posterior (arrowhead). (C) Cold-shock treatment did
not alter 
Tub37C localization (arrowhead). (D,E) Centers of MT
polymerization (arrowhead) were detected in the vicinity of the oocyte
nucleus, never at the posterior or at cortical regions after short recovery
times. (F) MTs appear along the anterior margin after 10 minutes
regrowth. (G) MT regrowth along the cortex in the DV orientation during
intermediate recovery times. (H) Optical section taken in the center of
the same oocyte as in G. Clouds of Khc positive dots at the ventral and dorsal
sides (arrowheads) represent transverse cuts of the cortical MTs. The AP
subset in the center was not yet established. (I,J) Optical section of
the same oocyte. Thirty minutes recovery at 25°C was sufficient to regrow
MTs to the control situation. Cortical DV subset (I, arrowhead) and, in the
center (J), MTs in the AP direction (arrow). Asterisk, oocyte nucleus. Scale
bar: 20 µm.
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