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Fig. 7. Correlation of the stage-dependent MT organization and grk as
well as osk mRNA localization from stage 7 through stage 9.
grk (green) and osk (blue) are visualized by fluorescence
in-situ hybridization. At stage 7 (left), grk and osk are
found mostly at the anterior cortex. grk is rapidly transported
toward the oocyte nucleus in the transition from stage 7 to stage 8,
exploiting the DV MT subset (black). The DV subset comprises long cortical MT
fibers as well as the shorter cortical fibers. osk mRNA localization
correlates with the successive progression of AP MT subset (red) formation.
osk is transported from the anterior toward the center following the
open diaphragm MTs in the center. By stage 9 (right), upon oocyte growth and
complete AP orientation of center MTs, osk is transported toward the
posterior.
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