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Fig. 1. Analysis of Cre expression in DBH-Cre mice. (A-C) Using the ROSA26 reporter mouse line, Cre-mediated lacZ expression was detected by ß-galactosidase staining in sympathetic ganglia of E16.5 mouse embryos. lacZ expression is demonstrated for the sympathetic chain (A,B) and is absent in sweat gland tissue (C). (D) Cre-mediated elimination of exon 16 in gp130DBHcre mice is revealed by the amplification of an 800 bp band from sympathetic ganglia (1), adrenal gland (2) and the absence in non-adrenergic tissue (tail-cut, 3). (A) Whole-mount staining; (B,C) ß-galactosidase staining of tissue sections.





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