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Fig. 2. Sweat gland innervation in gp130DBHcre mice lacks
cholinergic properties. Frozen sections from P60 mouse footpads of control
gp130fl/fl and mutant gp130DBHcre mice
were co-stained for (A-F) TUJ1 and VIP, (G-M) TUJ1 and VAChT,
(N-S) and TUJ1 and ChT1. Nuclei were stained with DAPI. The expression
of VIP-IR, VAChT-IR and ChT1-IR is virtually absent in sweat gland innervation
of gp130DBHcre mice, as is evident from the individual
stainings (F,M,S) and the quantitative analysis (bar graphs, left). By
contrast, TUJ1-IR fibers are not affected, as shown by the individual
stainings (E,L,R) and the overlays (D,K,Q). For the quantitative analysis,
sections were stained individually for VIP-IR, VAChT-IR and ChT1-IR (no
co-staining with TUJ1). Quantitative data are expressed as mean±s.e.m.
(n=3-8 for each group).
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