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Fig. 5. IL6 cytokine expression in mouse sweat gland tissue. (A,B) Individual sweat gland coils were dissected from P4 Toluidine-stained sections by LCM. (A) Before and (B) after dissection of sweat gland coil. (C) RT-PCR detected the expression of CNTF, CLC, CT1 and NP, but not of OSM and LIF, in P4 sweat glands, whereas all cytokines could be detected in total RNA from E15 mouse embryos. (D-F) In situ hybridisation of P10 sweat glands revealed expression of CLC (D), CLF (E) and CT1 (F). The in situ hybridization signal is mostly localized lateral to the secretory cells, and is most pronounced for CLF (E). (G) No signal was detected for NP, correlating with the minor band in the RT-PCR (see C). (H) GPA (2 ng/ml), CNTF (1.5 ng/ml), CLC/CLF (100 ng/ml) and NP (500 ng/ml) induce the expression of VIP in cultures of chick sympathetic neurons.





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