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Fig. 5. Colony formation from Sall1-mutant metanephric mesenchyme obtained from intercrosses of Sall1-GFP mice. (A-F) Colonies derived from Sall1+/+ (A,B), Sall1+/- (C,D) and Sall1-/- (E,F) mesenchyme were stained both with anti-GFP (A,C,E, green) and with anti-E-cadherin antibodies (B,D,F, red). Sall1-deficient colonies (E,F) were significantly smaller than those from wild-type (A,B) and heterozygous (C,D) mesenchyme. Scale bars: 50 µm.





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