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Fig. 6. PCP pathways regulate colony size and the differentiation of
colony-forming cells. (A) Immunostaining of activated JNK1 and 2 in
the colony. (B,C) The addition of two kinds of JNK inhibitors (JNKI1
and JNKI2, 10 µmol/l) gave rise to smaller colonies than did the control
without reagents or HIV-TAT peptide (10 µmol/l). (D) The
transduction of CA-Rac1 resulted in an increase in colony size,
whereas that of DN-Rac1 resulted in a decrease. (E,F) The
addition of Y27,632 (E, 10 µmol/l), or the transduction of
DN-RhoA, increased colony size, while activation with
CA-RhoA decreased it (F). (G,H) Activation of the
ß-catenin pathway by adding LiCl (G, 10 µmol/l) or transducing the
active form of ß-catenin (H) gave rise to smaller colonies.
(I) RT-PCR analysis of colonies treated with JNKI-1, HIV-TAT peptide,
JNKI-2, Y27,632 and LiCl. (J) E-cadherin expression was lost with
JNKI-1 and -2 but not with LiCl. EGFP transgenic mesenchyme was used for
B,C,E,G and J, while wild type was used for D,F and H, to visualize colonies
infected by retrovirus vectors. c, colony. Scale bars: 50 µm.
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