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Fig. 1. Fgf9, FGFR1 and FGFR2 expression in the embryonic cecum. (A,B) Whole-mount in situ hybridization was performed on isolated E11.5 (A) and E13.5 (B) wild-type GI tracts to detect Fgf9 expression. Insets show small intestine and colon. Fgf9 expression was uniformly expressed in the epithelium of the cecum, small intestine and colon. (C) Frozen section of an E13.5 whole-mount in situ hybridization showing Fgf9 expression in the cecal epithelium (arrows). (D-F) Immunohistochemical analysis of E12.5 cecal sections stained with an anti-FGFR1 (E) or anti-FGFR2 (F) polyclonal antibody. Control (D) staining, in which the primary antibody was absent, shows no signal. D',E' and F' are higher magnification views of the boxes in D,E and F, respectively. FGFR1 (E') and FGFR2 (F') are expressed in both cecal epithelium (black arrows) and mesenchyme (blue arrows). Co, colon; I, Ileum; SI, small intestine; Ce, cecum.





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