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Fig. 5. Effect of FGF9 and FGF10 on growth of cecal explants. E12.5
embryonic wild-type cecums were removed and embedded in Matrigel. BSA-, FGF9-
or FGF10-soaked heparin beads were placed one bead diameter from the cecal
explant. (A-C) During the first 12 hours, the cecal epithelium and
mesenchyme did not significantly extend toward either the FGF9- or
FGF10-soaked bead compared with the BSA-soaked control bead. (D-F)
After 84 hours, the cecal epithelium had expanded and extended toward the
FGF10 bead (E,E',H) compared to the BSA bead (D,D',G); however, no
mesenchymal growth was observed (red solid line in D',E',G,H). By
contrast, cecal mesenchyme showed significant growth accompanied by epithelial
expansion when placed adjacent to an FGF9 bead (F,F',I). Note the
significantly thickened mesenchymal regions in F' and I (line).
D',E' and F' show higher magnification brightfield views of
the explants in D, E and F, respectively. (G-I) H&E stained frozen
sections showing the histology of cultured cecal explants at 84 hours. Red
dashed lines indicate the border of mesenchyme and epithelium. The solid lines
in D'-F',G-I indicate the thickness of the cecal mesenchymal
layer.
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