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Fig. 5. p38 is transiently inactivated on the future aboral side of the embryo. (A) Western blot analysis for phospho-p38 at the indicated stages. P-p38 is indicated by an arrowhead. 8, 8 cell; 60, 60 cell. (B) Embryos stained with anti-P-p38 antibody (B1-B3) and Hoechst's dye (B4-B6) at the indicated timepoints post-fertilization. The cleared region is indicated by arrowheads. Images are internal projections of 10 sections from a confocal z-series. (C) A single embryo expressing Lvp38-GFP (0.015 pg/pl), imaged using a live confocal timecourse, is shown at the indicated timepoints post-fertilization (C1-C3). The cleared region is indicated by arrowheads. Images are internal projections of 20 sections. (D) Schematic depicting the experimental approach used in E. (E) A similar embryo to the one in C, but also labeled with DiI (red). (E1-E3) Internal projections of 25 sections at the indicated hpf. (E4-E6) The same embryo at late gastrula stage, with morphology (E4), skeleton (E5) and DiI labels shown (E6). The oral (O) and aboral (A) poles are indicated.





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