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Fig. 4. Posterior ectopic Msk affects MAPK expression. Late larval wings, anterior upwards, dorsal leftwards. Antigens and GFP are listed in the bottom left-hand corner, the expression genotype is listed in the bottom right-hand corner. (A-C) hsp70-driven expression of HSV epitope-tagged MAPK (hs:M) after a 1-hour induction. (B,C) en::msk is added to hs:M. Yellow boxed area in B is expanded in C. The epitope tag antigen is elevated where Msk is co-expressed (in the posterior, en domain in B). (C) Lamin D antigen (green) stains the nuclear envelope. Much of this MAPK is now in nuclei (arrows in C). Arrowheads in A,B indicate the AP boundary. (D-F) en::GFP (D) and en:GAL4 driving UAS:GFP and UAS:msk together (E,F). pMAPK expression that is normally associated with veins and margin (arrowheads in D) is suppressed in the posterior compartment wing pouch in E: compare non-suppressed anterior wing margin pMAPK (arrows in D,E) with suppressed posterior wing margin pMAPK and L5 pro-vein (arrowheads in D,E). The GFP expression domain expands towards the anterior (arrow in F). Magnifications are equal in A,B,D-F.

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