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Fig. 3. Inactivation of the Gata3 gene. (A) The Gata3 gene was targeted by insertion of loxP sites (grey triangles) flanking exon 4 (encoding the first zinc finger), followed by a cassette, flanked by frt sites (grey rectangles), containing a translation stop codon, an internal ribosome entry site (Ires) driving the GFP gene, and the neomycin selection gene (neo). The GFP and neo genes were followed by SV40 polyadenylation signals. The targeting construct additionally contained the thymidine kinase (tk) and dyphteria toxin A (DT-A) genes at the 5' and 3' ends, respectively, for selection against random genomic integration. Mice with the original targeted allele (Gata3^ex4GFP) were mated with the More-cre germline deleter strain (Tallquist and Soriano, 2000) to excise exon 4 and generate the Gata3^GFP allele (referred to as Gata3^- allele). The different alleles were detected by Southern blot analysis of BamHI-digested genomic DNA with the indicated probe. The exons are numbered according to Pandolfi et al. (Pandolfi et al., 1995). (B) Southern blot analysis of wild-type (+/+), Gata3^ex4GFP (ex4GFP/+) and Gata3^GFP (GFP/+) tail DNA digested with BamHI. (C) GFP expression of the targeted Gata3 gene in Gata3^GFP/+ embryo at E9.5. Broken line indicates the contour of the embryo. ba, branchial arches; op, otic placode; nd, nephric duct; va, vitelline artery.

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