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Fig. 6. Ectopic tubulogenesis and guidance defects in the Gata3^-/- mesonephros. Control Gata3^+/+ (+/+) or Gata3^+/- (+/-) embryos (A,C,E,G,I,M,O) and Gata3^-/- (-/-) (B,D,F,H,J,K,L,N,P) embryos were analyzed for nephric duct defects at E9.25 (A,B,K), E9.5 (C-J,L-N) and E10.5 (O,P). (A,B) Confocal analysis of GFP expression from the Pax2^GFP BAC transgene shows the initiation of aberrant nephric duct morphogenesis. (C,D) Extreme case of mesonephros hypercellularity revealed by immunohistochemistry with an anti-Pax2 antibody. (E,F) Gata3^-/- ectopic ducts expressed the nephric duct marker E-cadherin. (G,H) Same section as in E,F, showing the colocalization of E-cadherin with GFP expressed from the targeted Gata3 locus. (I,J) Whole-mount in situ hybridization with a Pax2 cRNA probe reveals the presence of multiple nephric ducts (nd) growing towards the ectoderm at the level of the mesonephric tubules (mst) in Gata3^-/- embryos. (K,L) Gata3^-/- embryos in which a nephric duct grew caudally showed a guidance defect, as revealed by whole-mount in situ hybridization with a Pax2 cRNA probe. (M,N) Immunohistochemistry with an anti-Pax2 antibody showed an abnormally large distance between the nephric duct and nephric cord in Gata3^-/- embryos. (O,P) Brn1 cRNA in situ staining of a representative E10.5 embryo in which the duct turned abruptly in the direction of the surface ectoderm (broken line) and fused with it. da, dorsal aorta; ec, ectoderm; ms, mesonephros; mst, mesonephric tubules; nc, nephric cord; nd, nephric duct.

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