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Fig. 2. X-TSK expression requires BMP signaling. Results of the animal cap
(AC) assays. (A-H) Animal caps were prepared from stage 8-9 embryos
injected with 0.5 ng Chordin (B,E), 0.5 ng Chordin + 1 ng
BMP4 (C,F), 1 ng truncated BMP-receptor (tBR) (H),
or none of the above (controls; A,D,G). ACs were harvested at the time
equivalent to stage 14 and hybridized with XK81 (A-C) or
X-TSK (D-H) probes. Chordin downregulated XK81
expression (B), which was rescued by BMP4 (C). The expression of
X-TSK was repressed by Chordin (E), but rescued by
BMP4 (F). (H) Truncated BMP receptors repressed the expression of
X-TSK. (I) X-TSK expression in a normal embryo at stage 17.
(J) RT-PCR analysis with ACs harvested at stages 14 (lanes 1-8) and 17
(lanes 9-16). ODC is used as an internal control. X-TSK
expression in the ACs was recovered by increasing amounts of BMP4.
BMP4 overexpression induced X-TSK expression at both stages. WE,
whole embryo; NI, non-injected ACs.
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