DEV02181 Supplementary Material

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• Supplemental Figure 1 - p class=bodytextfullout>Fig. S1. mDA progenitors expressing Aldh1a1 develop from the Wnt1-positive midbrain FP and BP (cephalic flexure). (A) Sagittal and (B) coronal sections of E9.5 wild-type embryos hybridized with probes for Wnt1, Aldh1a1 and Shh. Bright-field micrographs are shown on the left of the corresponding dark-field views.

• Supplemental Figure 2 -

  Fig. S2. The ectopic mDA neurons persist in the rostral hindbrain of En1^+/Wnt1 mutants throughout embryogenesis and in adulthood. (A) Sagittal sections of wild-type and En1^+/Wnt1 embryos at E15.5 hybridized with probes for Th and the dopamine transporter Slc6a3. (B) Th immunostaining of horizontal sections from adult wild-type and En1^+/Wnt1 mutant mice. Arrows indicate the ectopic mDA neurons. SNc, substantia nigra pars compacta; VTA, ventral tegmental area; RrF, retrorubral field.

• Supplemental Figure 3 -

  Fig. S3. Ectopic expression of Otx2 in the rostral hindbrain of En1^+/Otx2 mutant embryos at E11.5 leads to a caudal expansion of the Wnt1-expression domain but ventrally only within the mutant rostral hindbrain FP. Wild-type and En1^+/Otx2 mutant embryos were hybridized with probes for Th, Wnt1 and Otx2. White arrows indicate the ectopic expression domain. Top row represents bright-field views of the dark-field pictures shown below.