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Fig. 4. Wnt1 is necessary for terminal differentiation of Th-expressing mDA
precursors. (A) Consecutive sagittal sections of wild-type and
Wnt1-/- embryos at E9.5 (n=8) hybridized with
probes for En1 and Aldh1a1. (B) Sagittal sections of
wild-type and Wnt1-/- embryos at E11.5 (n=8)
hybridized with En1 or immunostained for RC2 (a radial glia marker,
red). (C) Fluorescent immunodetection of Th (green) and Pitx3 (red) on
sagittal sections of wild-type and Wnt1-/- mutant embryos
at E11.5 (n=3). The white square in B corresponds to the region of
the sections in C. Graph shows that the number of Th-positive cells was
drastically reduced in the Wnt1-/- mutant at this stage
(mean±s.d./s.e.m. from three sibling pairs: wild type,
1958±129/92 cells; Wnt1-/-,14±11/8 cells).
Triple asterisks indicate numbers that differ at P<0.0024, paired
t-test. (D) Schematic drawing of a cross-section through the
E11.5 ventral midbrain of wild-type mouse embryos, suggesting a possible role
of Wnt1 in the generation of Pitx3-expressing mDA neurons.
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