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Fig. 1. Npn2 is expressed in migrating neural crest cells. Npn2 expression was revealed by whole-mount in situ hybridization of 31 somite stage chick (A) and E10.0 (28 somite) mouse (C) embryos. Neural crest cells were immunostained with anti-HNK-1 in longitudinal sections (B) and anti-p75 in transverse sections (D-F) through the embryos shown in A and C. Plane of section is indicated on the whole-mount view. (E,F) Higher magnification view of the regions boxed in D. Arrowheads indicate migrating neural crest cells. Identical results were obtained at E9.5. a, anterior; p, posterior; dm, dermomyotome; sc, sclerotome; nt, neural tube. Scale bars: 0.1 mm in A-D; 0.01 mm in E,F.





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