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Fig. 5. Npn2 is cell autonomously required for neural crest cells to avoid
Sema3f in vitro. Neural tubes from 14 to 24 somite mouse embryos were
cultured on Thermanox coverslips coated with fibronectin and spotted with
AP-Sema-3F conditioned medium conjugated with anti-placental alkaline
phosphatase. Neural crest cells normally avoid immobilized Sema3f (A),
while neural crest cells lacking the Npn2 receptor migrate equally well on
fibronectin and Sema3f substrates (B,C; n=5 spots in three
separate experiments). Neural crest cells were labeled with anti-p75, while
spots (outlined in white) were visualized using anti-mouse IgG-Alexa 488.
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