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Figure 1


Fig. 1. Medaka smp belongs to a novel family of vertebrate genes. (A) Smp bears two highly conserved regions (HRI and HRII), two 14-3-3 consensus-binding domains and a nuclear-localization signal (NLS). (B) A phylogenetic tree of the smp family showing two smp groups: A and B. Hs, Homo sapiens; Mm, Mus musculus; Gg, Gallus gallus; Xl, Xenopus laevis; Dr, Danio rerio; Fr, Fugu rubripes; Tn, Tetraodon negroviridis; Ol, Oryzias latipes. (C) Western blot after co-immunoprecipitation of 14-3-3 or SKIIP with Ol-Smp and FAM53B. Co-immunoprecipitation reveals an interaction of SKIIP with human FAM53B, but not with Ol-Smp.





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